Phase-Contrast Microscopy
It is very much difficult to obtain
sufficient contrast when living material examined, which has a refractive index
not far from that of liquid. This microscope has special optical system which
makes it possible to distinguish unstained structures within a cell which
differ slightly in their refractive index or thickness. It has special
phase-contrast objective with phase contrast condenser. Appearances of specimen
varying degree of “darkness”.
Advantages
- · Observing living unstained cell
- · Revealing intracellular structures
Disadvantages
- · Inability to evaluate staining reactions.
- · Difficult to observe thick specimens/ distorted image for thick specimen.
(Note: Refractive index)
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